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KMID : 0379520040200010063
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한국독성학회지
2004 Volume.20 No. 1 p.63 ~ p.69
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Protective Effects of Chitosan on the Cadmium Cytotoxicity in Rat Glioma Cells
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Baik Yong-Ah
Lee Jeoung-Rae
Kim Kang-Deuk
Kim Hae-Won
Lee Han -Sol
Hur Jung-Mu
Oh Jay-Min
Choi Min-Kyu
Chung Yeun-Tai
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Abstract
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Casapse-3 protease is known as a key role of apoptotic enzyme, and caspase-3 activity is a central event that occurs upstream of DNA fragmentation during apoptosis. This study demonstrates that chitosan pretreatment inhibits cadmium-induced apoptosis by attenuating the activity of caspase-3. We also analyzed the protective effect of chitosan on DNA fragmentation induced by cadmium. Cadmium toxicity was examined by DNA fragmentation and nuclear condensation with Hoechst stain. Caspase-3 activities were increased cadmium treated group for 3 hours compared with control. When chitosan (150 mg/ml) was pretreated at 30 min before cadmium treatment, cadmium cytotoxicity was suppressed in a dose-dependent manner evaluated by DNA fragmentation and caspase activity. From these results, it is suggest that the protective effect of chitosan pretreatment against cadmium-induced cytotoxicity is mediated through inhibition of caspase-3 protease activation and DNA fragmentation.
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KEYWORD
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Cadmium, Cytotoxicity, Chitosan, Rat glioma cell
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